Journal: Molecular Metabolism

Article Title: Neurons undergo pathogenic metabolic reprogramming in models of familial ALS

doi: 10.1016/j.molmet.2022.101468

Figure Lengend Snippet: Key resources table.

Article Snippet: E. coli (HT115 (F-, mcrA, mcrB, IN(rrnD-rrnE)1, rnc14::Tn10(DE3 lysogen: lacUV5 promoter -T7 polymerase)) expressing empty vector (L4440) or different RNAi-encoding plasmids ( C. elegans RNAi Collection (Ahringer)) , Source BioScience , SCR_017064.

Techniques: Binding Assay, Sequencing, Expressing, Plasmid Preparation, Recombinant, Software

Journal: Alcoholism, clinical and experimental research

Article Title: Genomewide Association Study of Alcohol Dependence Identifies Risk Loci Altering Ethanol-response Behaviors in Model Organisms

doi: 10.1111/acer.13362

Figure Lengend Snippet: Speed of locomotion was measured at 10 and 30 minutes, and expressed as a percent of the untreated control speed. Control worms were tested simultaneously on the same plates. Worms were treated with 400 mM exogenous ethanol. The waxy cuticle of worms excludes most of the exogenous ethanol, tissue concentrations are approximately 12% of the exogenous dose (~48 mM). A, B, C: RNAi-induced gene knockdown reduced sensitivity to ethanol for C16E9.1 (a COL6A3 ortholog) but not for two other orthologous genes relative to untreated worms. D: Loss-of-function of the KLF12 ortholog, klf-3, prevented the development of acute functional tolerance between the 10- and 30-minute time points relative to wild-type N2. E, F: unc-68 mutant animals demonstrate reduced sensitivity to ethanol relative to wild-type N2. Statistical significance is shown for 2-way ANOVA followed by post-hoc comparisons across genotypes (*, p<0.05, **, p<0.01, ***, p<0.001).

Article Snippet: Briefly, cultures of bacteria containing RNAi vectors corresponding to genes C16E9.1, C18H7.1, cutl-23 or empty vector (L4440) (Geneservice, Cambridge, UK) were plated on NGM plates with 1mM IPTG, and allowed to grow at room temperature for 24 hours.

Techniques: Control, Knockdown, Functional Assay, Mutagenesis

Journal: Molecular Metabolism

Article Title: Neurons undergo pathogenic metabolic reprogramming in models of familial ALS

doi: 10.1016/j.molmet.2022.101468

Figure Lengend Snippet: Key resources table.

Article Snippet: E. coli (HT115 (F-, mcrA, mcrB, IN(rrnD-rrnE)1, rnc14::Tn10(DE3 lysogen: lacUV5 promoter -T7 polymerase)) expressing empty vector (L4440) or different RNAi-encoding plasmids ( C. elegans RNAi Collection (Ahringer)) , Source BioScience , SCR_017064.

Techniques: Binding Assay, Sequencing, Expressing, Plasmid Preparation, Recombinant, Software